Coelenterazine 400A is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission. Coelenterazine 400 a
For Western blots. femtoCHROMO AP uses a modified BCIP (5-Bromo-4-Chloro-3'-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.
Iodoacetamide is a commonly used alkylation agent for blocking thiols of proteins.Alkylation by iodoacetamide of free cysteines, following their reduction, results in thecovalent coupling of a carbamidomethyl group (57.07Da) and prevents formation ofdisulfide bonds/ bridges.
Phenyl-β-D-galactopyranoside is a noninducing β-galactosidase substrate. It is used in selection of mutants of lacZ plasmid-based transgenic mice. It is an acceptor substrate in fucosyltransferase detection.