タンパク質の精製・発現
現在の絞り込み条件
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HCR IHC 二次抗体プローブ
HCR IHC 二次抗体プローブは、免疫組織化学染色(IHC：Immunohistochemistry)用ロバ由来二次抗体です。
標的タンパク質に特異的に結合するように設計された一次抗体（お客様にてご準備）とAmplifier(蛍光色素標識済ヘアピンDNA・別売)を組み合わせることで、組織中の標的タンパク質の局在を可視化できます。86-5412-89, 86-5412-90, 86-5412-91 他
24種類の製品があります
標準価格：46,800円〜
WEB価格：
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101Bio Ponceau S Stain 250 ml P5W4
Description:101Bio Ponceau S Stain can rapid and reversible stain Western Blot membrane for locating protein bands and comparing the protein bands intensity. It produces pink reddish stained bands on Western blot membranes. The stain is very useful because it does not have a deleterious effect on the property of proteins. It is also a common method for locating polypeptides on Western blots for protein sequencing. The stain binds to proteins on the membrane reversibly and works well for nitrocellulose and PVDF membranes. It is not recommended for positively charged membranes such as nylon membrane.Ready-to-use.
1種類の製品があります
標準価格：28,000円
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101Bio High Efficiency Protein Precipitation kit 30 ml / 30 ml P5W6
Description:Protein precipitation is an option for concentrating proteins and removing interfering substances found in protein samples such as salts, lipid and other components that may interfere with downstream applications. One of the most commonly used methods is trichloroacetic acid (TCA) precipitation. This is relatively simple and effective. However, proteins precipitated by TCA method is usually denatured and in many cases the solubility of protein is reduced. Another major disadvantage of traditional TCA/acetone precipitation method is its low efficiency for the samples of low protein concentration. The higher protein concentration sample can be precipitated much more effectively than the lower protein concentration samples. In order to overcome these shortcomings, we have developed this High Efficiency Protein Precipitation Kit, which is a modification of traditional TCA method. It is simple, rapid and highly effective. Low protein concentration samples can be effectively precipitated and concentrated in about 25-30 minutes. The precipitated protein can be re-dissolved readily in detergent containing buffers.Simple, rapid and easier to use than other kits
especially useful for precipitation of low protein concentration samples.All steps performed at room temperature.Done in 25 minutes.1種類の製品があります
標準価格：48,000円
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Detergent-free Exosomal Protein Extraction Kit 10 rxn P201
Description:This kit is for rapidly extract detergent-free total proteins from exosome in less than 5 minute. The protein extraction buffers do not contain any detergent and EDTA. The whole procedure is less than 5 minutes.The extracted protein can be used for proteomics (LC/MS), IP, ELISA, 2D-gel analysis, isoelectric focusing, SDS-PAGE, immunoblottings, and other applications.
1種類の製品があります
標準価格：97,000円
WEB価格：
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101Bio HRP Substrate Kit 30 ml / 30 ml P5W1
Description:101Bio HRP substrate kit provides high intensity signal and low background detection of low abundance endogenous proteins. This highly sensitive ECL substrate is available in a two-bottle format for Western blotting with horseradish peroxidase (HRP) enzyme conjugates. This high-performance substrate will pick up your most difficult-to-detect proteins in Western Blots. The ECL substrate is compatible with different membranes, blocking reagents and a wide range of antibody dilutions. 101Bio HPR substrate kit provides excellent performance, versatility and long-lasting signal for high quality data.Ready-to-use,Strong - pick up your most difficult-to-detect proteins,long-lasting signal (>4h),low background
1種類の製品があります
標準価格：46,800円
WEB価格：
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101Bio Antibody Enhancer 30 ml P5W2
Description:101Bio Antibody Enhancer can significantly increase the sensitivity of commonly used immunoassays such as Western blotting, ELISA and immunohistochemistry. The enhancer can reduce the amount of primary and secondary antibodies used due to higher antibody dilution employed, a significant saving of expensive antibodies. The signal intensity can be increased several folds in immunostaining applications with chromogenic or ECL substrates.Ready-to-use,Save expensive antibodies,Enhance signal intensity,BSA and preservative-free,long shelf life (2 years at 4oC).
1種類の製品があります
標準価格：46,800円
WEB価格：
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101Bio WB Stripping Solution 250 ml P5W3
Description:101Bio WB Stripping Solution (stripping buffer) is designed to rapidly strip antibodies bound to antigens on Western blotting membranes using chemiluminescent substrate. One of the major advantages of chemiluminescent detection is high sensitivity and the ability to strip reagents from a blot and re-probe the same blot. Re-probing Western blots are very useful for optimizing the blotting conditions such as testing different antibody dilution and membrane blocking conditions. The key to this process is to use conditions that cause the release of antibody from the antigen without causing a significant amount of antigen to be released from the membrane. During the stripping procedure, small amount of antigen is inevitably lost from the membrane. It is important to minimize this loss by stripping the antibody under gentle conditions.101Bio WB stripping solution can achieve maximum removal of antibodies from a membrane and preserving the integrity of the antigen in 12-15 minutes without using harsh conditions such as boiling and treated with strong reducing agents. A blot can be stripped and re-probed multiple times to visualize multiple proteins or to optimize experimental conditions without the need for running multiple gels.Ready-to-use,Short incubation time (12-15 minutes),Gentle stripping conditions,long shelf life (2 years at RT).
1種類の製品があります
標準価格：46,800円
WEB価格：
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101Bio Peroxidase Suppressor 30 ml P5W5
Description:101Bio Peroxidase Suppressor is designed to inhibit endogenous peroxidase activity commonly encountered in immunohistochemistry (IHC) procedures. Inhibiting endogenous peroxidase activity is essential for avoiding false positive and reduce the background of IHC. Many chemical agents such as H2O2 and sodium azide have been used for this purpose but the inhibition is usually incomplete. 101Bio Peroxidase Suppressor is a mixture of several potent peroxidase inhibitors and can completely inhibit endogenous peroxidase activity. The suppressor can be used in regular IHC as well as HRP-mediated super sensitive procedures such as tyramide signal amplification (TSA).Ready-to-use,Irreversible inhibition of endogenous peroxidase activity,Significantly reduce the background of sensitive assays using HRP,Very stable at RT.
1種類の製品があります
標準価格：41,400円
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101Bio Red Blood Cell Lysis Buffer 250 ml P5W7
Description:Red blood cells (RBC) are abundant in blood-rich organs such as spleen of mouse and are the major component of blood. In many experiments such as protein and nucleic acid extraction from blood rich organs and analysis of nucleated cells from blood by flow cytometry, the RBC are not desirable and should be removed as much as possible prior to the extraction procedures. 101Bio RBC Lysis Buffer is a modified ammonium chloride solution that can effectively lyse RBC without damaging other nucleated cells.The RBC lysis buffer is mainly used for lysis of RBC from mouse and human samples, such as whole blood (EDTA-treated), bone marrow, buffy coats and blood/RBC-rich organs (eg. spleen, liver, etc.).Simple, rapid and easy to use.Effectively lyse RBC, gentle on other cells.Done in 15-20 minutes !
1種類の製品があります
標準価格：43,200円
WEB価格：
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101Bio WB Blocking Solution 250 ml P5W8
Description:This WB blocking solution is designed to block non-specific binding of antibodies to Western Blot (WB) membranes. This proprietary formulated blocker is a non-casein and non-milk based solution that effectively blocks non-specific binding rapidly and effectively resulting in clear background, increased contrast and enhanced signal intensity. This solution can also be used in ELISA, IHC and other detection system where blocking of non-specific antibody binding is needed. This blocking solution does not contain detergent and Tris base (Trizma).Clear background,Increased contrast,Enhanced signal intensity,No detergent, no Tris base.
1種類の製品があります
標準価格：43,200円
WEB価格：
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101Bio Denaturing Protein Solubilization Reagent 10 ml P5W9
Description:This reagent is formulated for dissolving protein pellets extracted using protein kits (such as 101Bio Cat.#: P503, P511, P513, P518 and P523 kits). The solubilized proteins are denatured due to the presence of an anionic detergent (SDS). Proteins dissolved in this reagent is mainly used for SDS-PAGE electrophoresis and Western blotting. In some cases, the solubilized protein can also be used for immunoprecipitation and trypsin digestion if SDS is removed using our SDS-Remover (Cat. # P5W12).
1種類の製品があります
標準価格：32,400円
WEB価格：
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101Bio Non-Denatured Protein Solubilization Reagent 10 ml P5W10
Description:This reagent is formulated for dissolving protein pellets using protein kits (such as 101Bio Cat.#: P503, P511, P513, P518 and P523 kits). The solubilized proteins are non-denatured and can be used for ELISA, immunoprecipitation/Co-IP, enzymatic activity determination and other applications.
1種類の製品があります
標準価格：32,400円
WEB価格：
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101Bio Protein Solubilization Reagent for MS 2 ml P5W11
Description:This reagent is formulated for dissolving protein pellets extracted using protein kits (such as 101Bio Cat.#: P503, P511, P513, P518 and P523 kits). The solubilized proteins are denatured due to the presence of SDS and can be used for trypsin digestion and for subsequent mass spectrometry analysis.
1種類の製品があります
標準価格：32,400円
WEB価格：
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101Bio SDS-Remover 10 ml P5W12
Description:Sodium dodecyl sulfate (SDS) is one of the most commonly used detergents for biological research. However, the presence of SDS in the sample could interfere with downstream applications. For instance, SDS must be reduced to below certain concentration before trypsin digestion of protein can be performed effectively. The presence of SDS in protein sample could interfere with mass/MS analysis and needs to be removed prior to the analysis. The presence of SDS in protein samples also interferes with antigen-antibody binding and has a negative impact for experiments such as immunoprecipitation and ELISA. This proprietary formulated reagent is designed for easy and rapid removal of SDS in solution by precipitation. The efficacy is superior to SDS-removal column and TCA precipitation with minimum protein loss (<20%). Due to small volume of SDS-Remover used, the final protein concentration of the sample is not significantly impacted.
1種類の製品があります
標準価格：37,800円
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Albumin Depletion Reagent for Plasma and Serum (10 ml) NEW! 10 rxn P5W13
Description:Human/animal serum and plasma are the best source of samples for biomarker identification. However, the presence of large amount of albumin interferes with many assays and functional proteomic analysis. Serum albumin accounts for 50-70% of the total serum protein that causes loss of resolution for 1D and 2D electrophoresis. Effective removal of albumin using our reagent can increase sensitivity of the assay for many folds. Unlike resin-based depletion kit (that only deals with microliter amount of sample), Minute albumin depletion reagent can handle a few microliters to several ml of samples. Over 90% of albumin can be removed in less than 5 min. This product is for research use only.
1種類の製品があります
標準価格：43,200円
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3-min Detergent-free Total Protein Extraction Kit (Animal Tissues) 5 rxn P506S
1種類の製品があります
標準価格：40,500円
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3-min Detergent-free Total Protein Extraction Kit (Animal Cells) 50 rxn P505L
1種類の製品があります
標準価格：128,000円
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3-min Detergent-free Total Protein Extraction Kit (Animal Tissues) 50 rxn P506L
1種類の製品があります
標準価格：112,000円
WEB価格：
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3-min Detergent-free Total Protein Extraction Kit (Animal Cells) 5 rxn P505S
1種類の製品があります
標準価格：41,000円
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Long-term Cell Tracer 580 (Green) 1 ml P710G
Description:Cell Tracers are a class of organic fluorescent dots with comparable size and photostability to inorganic quantum dots (QDs) to overcome the limitations of quantum dots (e.g., potential toxicity and compromised fluorescence in presence of ROS) in advanced bio-imaging applications. Upon conjugation with a cell penetrating peptide, Cell Tracer show excellent labeling efficiency to living cells and outperform the current gold standard, inorganic quantum dots cell labeling kits, in long-term in vitro/in vivo cell tracing applications.Stem cell tracing study also suggests that Cell Tracer has no negative effect on mesenchymal stem cell differentiation. The merits of Cell Tracer make them promising alternatives to quantum dot probes, which is of high importance for translational research applications.Additionally, the biocompatible polymeric matrix endows this kind of organic fluorescent dots customized surface functional groups for further modification/conjugation with a variety of biomolecules for specific imaging tasks besides cell tracking.Long term photostability - 3 weekes in vivo Polymeric dots, nonviral system, direct labeling, no viral footprint High brightness - high signal to noise ratio Different colors - blue, green, yellow, red Low toxicity - no heavy metal elements.Customization friendly - can conjugate with desired biomolecules.easy to use - only one component
1種類の製品があります
標準価格：135,000円
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Long-term Cell Tracer 580 (Green) sample 0.1 ml P710GS
Description:Cell Tracers are a class of organic fluorescent dots with comparable size and photostability to inorganic quantum dots (QDs) to overcome the limitations of quantum dots (e.g., potential toxicity and compromised fluorescence in presence of ROS) in advanced bio-imaging applications. Upon conjugation with a cell penetrating peptide, Cell Tracer show excellent labeling efficiency to living cells and outperform the current gold standard, inorganic quantum dots cell labeling kits, in long-term in vitro/in vivo cell tracing applications.Stem cell tracing study also suggests that Cell Tracer has no negative effect on mesenchymal stem cell differentiation. The merits of Cell Tracer make them promising alternatives to quantum dot probes, which is of high importance for translational research applications.Additionally, the biocompatible polymeric matrix endows this kind of organic fluorescent dots customized surface functional groups for further modification/conjugation with a variety of biomolecules for specific imaging tasks besides cell tracking.Long term photostability - 3 weekes in vivo Polymeric dots, nonviral system, direct labeling, no viral footprint High brightness - high signal to noise ratio Different colors - blue, green, yellow, red Low toxicity - no heavy metal elements.Customization friendly - can conjugate with desired biomolecules.easy to use - only one component
1種類の製品があります
標準価格：48,000円
WEB価格：
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Long-term Cell Tracer 580 (Yellow) 1 ml P710Y
Description:Cell Tracers are a class of organic fluorescent dots with comparable size and photostability to inorganic quantum dots (QDs) to overcome the limitations of quantum dots (e.g., potential toxicity and compromised fluorescence in presence of ROS) in advanced bio-imaging applications. Upon conjugation with a cell penetrating peptide, Cell Tracer show excellent labeling efficiency to living cells and outperform the current gold standard, inorganic quantum dots cell labeling kits, in long-term in vitro/in vivo cell tracing applications.Stem cell tracing study also suggests that Cell Tracer has no negative effect on mesenchymal stem cell differentiation. The merits of Cell Tracer make them promising alternatives to quantum dot probes, which is of high importance for translational research applications.Additionally, the biocompatible polymeric matrix endows this kind of organic fluorescent dots customized surface functional groups for further modification/conjugation with a variety of biomolecules for specific imaging tasks besides cell tracking.Long term photostability - 3 weekes in vivo Polymeric dots, nonviral system, direct labeling, no viral footprint High brightness - high signal to noise ratio Different colors - blue, green, yellow, red Low toxicity - no heavy metal elements.Customization friendly - can conjugate with desired biomolecules.easy to use - only one component
1種類の製品があります
標準価格：135,000円
WEB価格：
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Long-term Cell Tracer 580 (Yellow) sample 0.1 ml P710YS
Description:Cell Tracers are a class of organic fluorescent dots with comparable size and photostability to inorganic quantum dots (QDs) to overcome the limitations of quantum dots (e.g., potential toxicity and compromised fluorescence in presence of ROS) in advanced bio-imaging applications. Upon conjugation with a cell penetrating peptide, Cell Tracer show excellent labeling efficiency to living cells and outperform the current gold standard, inorganic quantum dots cell labeling kits, in long-term in vitro/in vivo cell tracing applications.Stem cell tracing study also suggests that Cell Tracer has no negative effect on mesenchymal stem cell differentiation. The merits of Cell Tracer make them promising alternatives to quantum dot probes, which is of high importance for translational research applications.Additionally, the biocompatible polymeric matrix endows this kind of organic fluorescent dots customized surface functional groups for further modification/conjugation with a variety of biomolecules for specific imaging tasks besides cell tracking.Long term photostability - 3 weekes in vivo Polymeric dots, nonviral system, direct labeling, no viral footprint High brightness - high signal to noise ratio Different colors - blue, green, yellow, red Low toxicity - no heavy metal elements.Customization friendly - can conjugate with desired biomolecules.easy to use - only one component
1種類の製品があります
標準価格：48,000円
WEB価格：
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Sample of P201 2 rxn P201S
Description:This kit is for rapidly extract detergent-free total proteins from exosome in less than 5 minute. The protein extraction buffers do not contain any detergent and EDTA. The whole procedure is less than 5 minutes.The extracted protein can be used for proteomics (LC/MS), IP, ELISA, 2D-gel analysis, isoelectric focusing, SDS-PAGE, immunoblottings, and other applications.
1種類の製品があります
標準価格：30,000円
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sRNA-into-Exosome Kit (Chemical) 20 rxn P401
Description:ExoFectin sRNA-into-Exosome Kit (chemical) is a unique blend of polymers designed for the delivery of small RNAs including miRNA and siRNA into exosomes. This transfection reagent provides highly efficient loading abilities of miRNA or siRNA into exosomes, allowing modified exosomes to carry and deliver small RNAs into target cells. This kit provides the following advantages:efficient gene delivery,gentle on treated exosomes,no small RNA aggregation in exosome.
1種類の製品があります
標準価格：136,000円
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Easy-Exo Saliva Exosome Isolation Kit, 95% pure 20 rxn P530
Description:Diagnoses using exosomes derived from saliva have been attracting great attention in recent years because of the ease of sample collection. However, saliva samples are challenging when it comes down to exosome isolation. In addition to cells and cell debris, large amount of amylase, mucin and glycoprotein is present in saliva, making the sample viscous and hard to manipulate. Pretreated such as sonication and dilution, are often required. In many cases, the saliva samples can still be difficult to handle even after pre-treatment. This kit is specifically designed to address these issues using the proprietary saliva filters. Highly viscous saliva can be converted to non-viscous solution instantly by passing the sample through the filter. Exosomes can then be readily isolated from as little as 100 µl saliva using highly effective polysaccharide method.Easy - No sonication for treating viscous saliva.High efficiency - only require 100-600 µl sample.
1種類の製品があります
標準価格：100,000円
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Exosome Isolation kit (for cell culture media) 10 rxn P100
Description:P100 kit is for exosome purification / isolation at high yield and purity from cell culture media.Easy to use: No ultra-centrifugation (< 2 hours).10 fold higher yield (vs. other kits and ultracentrifuge).Save cost (vs. antibodies-beads method)
Isolate Pure exosome (>95% purity vs. 25% purity by exosome precipitation method).Intact exosome (good morphology).Use as little as 2 ml cell media to achieve high yield of exosomes for any downstream applications: EM study, exosome label, exosome subpopulation, qRT-PCR profiling of exosomal miRNAs, and gel analysis of exosomal proteins.1種類の製品があります
標準価格：81,000円
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