Magenta-gal is used as an alternative to X-gal that produces a red color. Magenta-Gal is hydrolyzed by β-galactosidase to produce a red precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce red colonies when grown on plates containing magenta-gal.Magenta-gal is commonly used in conjunction with IPTG for red/white screening.
5-Bromo-6-chloro-3-indoxyl β-D- galactopyranoside
MOLECULAR BIOLOGY GRADE
Storage/Handling: Store at -15°C in a dark, dry place.