83-2965-06　AMV-RT High Spec Activity 500U　RTHSA5

特徴

• AMV RT-XL is a superior grade of native reverse transcriptase isolated from Avian Myeloblastosis virus.  This enzyme synthesizes cDNA from single-stranded RNA.   Selected for very high specific activity, concentration and purity. Applications: cDNA Library production, First strand cDNA synthesis for cloning, RNA sequencing, RT-PCR, High-temperature cDNA Synthesis at up to 50ºC.
• Reagent supplied, with a protocol for cDNA synthesis:  AMV Reverse Transcriptase 10X Reaction Buffer (Catalog # ARB 45).
• At 1X concentration the magnesium ion concentration is only 5 mM.
• Under this condition, endogenous RNaseH activity is not active.
• Unit Definition:  One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37ºC using  poly(rA)-d(T)12-18 as template primer.
• Unit Assay Conditions: 0.5 mM [3H]-TTP, 0.4 mM rAn.d(T)12-18, 50 mM TrisHCl, pH 8.3, 40 mM KCl, 6.0 mM MgCl2, and 4.0 mM DTT.  AMV RT preparations are diluted in a dilute phosphate buffer and added to the pre-incubated reaction to give linear kinetics.
• 50% Glycerol based storage buffer.
• Storage Temperature: -70ºC