83-2965-01　AMV Reverse Transcriptase 5,000 U　AMV007-5

特徴

• AMV Reverse Transcriptase (Standard Grade) synthesizes cDNA from single stranded RNA. The standard concentration is 20 units/µL and the specific activity is greater than 40,000 units per mg.
• It is qualified for cDNA synthesis and RT-PCR.
• Applications: cDNA Library production, First strand cDNA synthesis for cloning, RNA sequencing, RT-PCR, High-temperature cDNA Synthesis at up to 50ºC.
• Reagent supplied, with a protocol for cDNA synthesis:  AMV Reverse Transcriptase 10X Reaction Buffer (Catalog # ARB 45).
• At 1X concentration the magnesium ion concentration is only 5 mM.
• Under this condition, endogenous RNaseH activity is not active.
• Unit Definition:  One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37ºC using  poly(rA)-d(T)12-18 as template primer.
• Unit Assay Conditions: 0.5 mM [3H]-TTP, 0.4 mM rAn.d(T)12-18, 50 mM TrisHCl, pH 8.3, 40 mM KCl, 6.0 mM MgCl2, and 4.0 mM DTT.  AMV RT preparations are diluted in a dilute phosphate buffer and added to the pre-incubated reaction to give linear kinetics.
• Storage Temperature: -70ºC AMV RT stored in a Trehalose-based storage buffer, is identical to the standard glycerol-based storage buffer except that 1.0 M Trehalose is substituted for 50% glycerol and Specific Activity is greater than 50,000 units per mg.  This product is suited for the development of lyophilized preparations.  Recommended storage temperature is -70ºC.