Titan HotTaq Probe qPCR Universal Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform singleplex or duplex qPCR, with the exception of template, primers, and probes.
The qPCR Mix contains optimized components and Titan HotTaq DNA polymerase supplied in a proprietary reaction buffer that enables efficient amplification of regular and GC-rich targets.
Titan HotTaq Probe qPCR Universal Mix is optimized for DNA/LNA hydrolysis probes based on the 5' flap endonuclease activity.
Suited for DNA/LNA hydrolysis probe-based assays, detection and quantification of DNA and cDNA targets, profiling gene expression, microbial detection, viral load determination.
仕様
サイズ：250 rxn
Size： 250 rxn （1 ml）
Quality control： Free of detectable, non-specific nucleases
Reagent composition：
•Titan HotTaq DNA polymerase
•Titan HotTaq Probe qPCR Universal buffer
•15 mM MgCl2 ： 1x PCR solution – 3 mM MgCl2
•dNTPs, including dUTP. The mix allows UNG treatment to prevent carryover contamination from previous runs.
•Internal reference based on ROX dye.
IMPORTANT： UNG is not included in the Titan HotTaq Probe qPCR Universal Mix and must be purchased separately.
