Titan HotTaq Power Mix is a premixed ready to load solution containing： all reagents required for PCR （except template, primers and water）, compound needed for direct loading onto agarose gel and two tracking dyes to follow the electrophoresis.
Titan HotTaq Power Mix contains two enzymes – Titan HotTaq DNA polymerase and a proofreading polymerase.These enzymes together have both the 5'→ 3' exonuclease activity as well as the 3'→ 5' proofreading activity.
Suited for HotStart PCR and proofreading.
仕様
サイズ：250 rxn
Size： 250 rxn （1 ml）, concentration （MgCl2） 12.5 mM
Unit definition： One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74oC.
Quality control： Free of detectable, non-specific nucleases
Reagent composition：
•Titan HotTaq DNA Polymerase
•Proofreading enzyme
•5x Reaction Buffer
•12.5 mM MgCl2： 1x PCR solution – 2.5 mM MgCl2
•2 mM dNTPs of each： 1x PCR solution – 200 μM dATP, 200 μM dCTP, 200 μM dGTP and 200 μM dTTP
•BSA
•Blue dye： Migration equivalent to 3.5-4.5 kb DNA fragment
•Yellow dye： Migration rate in excess of primers in 1％ agarose gel： ＜35-45 bp
•Compound that increases sample density for direct loading
