Titan HotTaq Power Mix is a premixed ready to use solution containing: all reagents required for PCR (except template, primers and water).
Titan HotTaq Power Mix contains two enzymes – Titan HotTaq DNA polymerase and a proofreading polymerase.These enzymes together have both the 5'→ 3' exonuclease activity as well as the 3'→ 5' proofreading activity.
Suited for HotStart PCR and proofreading.
仕様
サイズ:250 rxn
Size: 250 rxn (1 ml), concentration (MgCl2) 12.5 mM
Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74oC.
Quality control: Free of detectable, non-specific nucleases
Reagent composition:
•Titan HotTaq DNA Polymerase
•Proofreading enzyme
•5x Reaction Buffer
•12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
•2 mM dNTPs of each: 1x PCR solution – 200 μM dATP, 200 μM dCTP, 200 μM dGTP and 200 μM dTTP