Titan Taq 5x PCR Mix Ready to Load is a premixed ready-to-use solution containing： all reagents required for PCR （except template, primers and water）, additional compound needed for direct loading onto agarose gel and two tracking dyes （blue and yellow） that allow to monitor progress during electrophoresis.
Suited for a wide range of PCR assays.
仕様
サイズ：250 rxn
Size： 250 rxn （1 ml）
Unit definition： One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74oC.
Quality control： Free of detectable, non-specific nucleases
Reagent composition：
•Titan Taq DNA polymerase
•5x Reaction Buffer 1： 0.4 M Tris-HCl, 0.1 M （NH4）2SO4, 0.1％ w/v Tween-20
•12.5 mM MgCl2： 1x PCR solution – 2.5 mM MgCl2
•1 mM dNTPs of each： 1x PCR solution – 200 μM dATP, 200 μM dCTP, 200 μM dGTP and 200 μM dTTP
•Blue dye： Migration equivalent to 3.5-4.5 kb DNA fragment
•Yellow dye： Migration rate in excess of primers in 1％ agarose gel： ＜35-45 bp
•Compound that increases sample density for direct loading
