Titan Taq 5x PCR Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).
Suited for a wide range of PCR assays and TA cloning
仕様
サイズ:250 rxn
Size: 250 rxn (1 ml)
Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74oC.
Quality control: Free of detectable, non-specific nucleases
Reagent composition:
•Titan Taq DNA polymerase
•5 x Reaction Buffer 1: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
•12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
•1 mM dNTPs of each: 1x PCR solution – 200 μM dATP, 200 μM dCTP, 200 μM dGTP and 200 μM dTTP