Atlas H Minus M-MLV Reverse Transcriptase is a genetically modified M-MLV RT which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity.
This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates.
Removal of the RNase H activity results in an increase of full-length cDNA products.
The enzyme has RNA polymerization-dependent and DNA polymerization-dependent activity but lacks ribonuclease H activity.
仕様
サイズ：10000 U
Concentration： 200 U/μl
Unit definition： One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37oC.
Quality control： Free of detectable, non-specific nucleases
Supplied with：
•Atlas H Minus M-MLV Reverse Transcriptase
•5x RT Reaction Buffer - 250 mM KCl, 15 mM MgCl2, 10 mM DTT, 100 mM Tris-HCl （pH 8.4）.
