特徴
- Titan HotTaq DNA Polymerase is a chemically modified Titan Taq DNA Polymerase.
- At ambient temperatures it is inactive, having no polymerization activity.
- Titan HotTaq DNA Polymerase is activated by a 15 min incubation step at 95°C.
- This prevents extentension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup.
- The enzyme has 5'→3' polymerization-dependent exonuclease replacement activity but lacks 3'→ 5' exonuclease activity.
- Suited for Hot Start PCR, DHPLC and TA cloning.
仕様
- サイズ:500 U
- Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
- Concentration: 5 U/μl
- Quality control: Free of detectable, non-specific nucleases
- Supplied with:
- •Titan HotTaq DNA Polymerase
- •10x Reaction Buffer B1 (Mg2+, detergent free): Tris-HCl and (NH4)2SO4
- •10x Reaction Buffer B2 (Mg2+ free): Tris-HCl, (NH4)2SO4 and detergent
- •25 mM MgCl2
- •10x Enhancer : Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). Enhancer should be used at a defined working concentration (1x, 2x or 3x solution).
- Enhancer is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.
- 研究専用試薬です。本製品の使用は、研究用に限定して販売しております。医薬品の製造、診断、臨床検査用途には使用できません。
- 【試薬に関するお問合せ】
- アズワン株式会社 試薬・プロセス材料グループ
- TEL:06-6447-8641
- FAX:06-6447-8642
- E-mail:[email protected]
商品のバリエーション (サイズ違い・スペック違い・オプション品など)
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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83-2416-38
Titan HotTaq DNA polymerase 1000 U
|
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83-2416-37
Titan HotTaq DNA polymerase 500 U
|
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掲載カタログ情報
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