背景：Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
仕様
サイズ：20µg
種由来：virus
発現宿主：Baculovirus-Insect Cells
純度：> 85 % as determined by reducing SDS-PAGE.
遺伝子構造：A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (YP_009724397.2) (Met1-Ala419(335Gly/Ala)) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
エンドトキシン濃度：< 1.0 EU per µg as determined by the LAL method.
分子量：47.08 kDa
組成：Lyophilized from sterile PBS, 500 mM NaCl, pH 7.0.
保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
