89-8147-06 Recombinant 2019-nCoV Nucleocapsid Protein, Biotinylated (His Tag) 20ug PKSR030512
[Elabscience]
特徴
- 背景：Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
仕様
- サイズ：20µg
- 種由来：virus
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 85 % as determined by reducing SDS-PAGE.
- 遺伝子構造：A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (YP_009724397.2) (Met1-Ala419(335Gly/Ala)) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
- 別名（遺伝子名）：NP;2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein
- アミノ酸配列：(Met1-Ala419(335Gly/Ala))
- タグ種類：C-6His
- アクセッションＮｏ．：YP_009724397.2
- エンドトキシン濃度：< 1.0 EU per µg as determined by the LAL method.
- 分子量：47.08 kDa
- 組成：Lyophilized from sterile PBS, 500 mM NaCl, pH 7.0.
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com//viewpdf-376358-Elabscience-PKSR030512.pdf
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