測定原理：This ELISA kit uses the Sandwich-ELISAprinciple. The micro ELISA plate provided in this kit has been pre-coated withan antibody specific to Human NGAL. Samples (or Standards) and biotinylateddetection antibody specific for Human NGAL are added to the micro ELISA platewells. Human NGAL would combined with the specific antibody. ThenAvidin-Horseradish Peroxidase (HRP) conjugate are added successively to eachmicro plate well and incubated. Free components are washed away. The substratesolution is added to each well. Only those wells that contain Human NGAL, biotinylateddetection antibody and Avidin-HRP conjugate will appear blue in color. Theenzyme-substrate reaction is terminated by the addition of stop solution andthe color turns yellow. The optical density (OD) is measuredspectrophotometrically at a wavelength of 450 ± 2 nm. The OD value isproportional to the concentration of Human NGAL. You can calculate theconcentration of Human NGAL in the samples by comparing the OD of the samplesto the standard curve.
仕様
サイズ：96T
測定タイプ：Sandwich
検出方法：Colormetric
感度：0.06ng/mL
測定範囲：0.13~8ng/mL
測定原理：This ELISA kit uses the Sandwich-ELISAprinciple. The micro ELISA plate provided in this kit has been pre-coated withan antibody specific to Human NGAL. Samples (or Standards) and biotinylateddetection antibody specific for Human NGAL are added to the micro ELISA platewells. Human NGAL would combined with the specific antibody. ThenAvidin-Horseradish Peroxidase (HRP) conjugate are added successively to eachmicro plate well and incubated. Free components are washed away. The substratesolution is added to each well. Only those wells that contain Human NGAL, biotinylateddetection antibody and Avidin-HRP conjugate will appear blue in color. Theenzyme-substrate reaction is terminated by the addition of stop solution andthe color turns yellow. The optical density (OD) is measuredspectrophotometrically at a wavelength of 450 ± 2 nm. The OD value isproportional to the concentration of Human NGAL. You can calculate theconcentration of Human NGAL in the samples by comparing the OD of the samplesto the standard curve.
