測定原理：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided inthis kit has been pre-coated with an antibody specific to Human TF. Samples (orStandards) and biotinylated detection antibody specific for Human TF are addedto the micro ELISA plate wells. Human TF would combined with the specificantibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are addedsuccessively to each micro plate well and incubated. Free components are washedaway. The substrate solution is added to each well. Only those wells thatcontain Human TF, biotinylated detection antibody and Avidin-HRP conjugate willappear blue in color. The enzyme-substrate reaction is terminated by theaddition of stop solution and the color turns yellow. The optical density (OD)is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD valueis proportional to the concentration of Human TF. You can calculate theconcentration of Human TF in the samples by comparing the OD of the samples tothe standard curve.
仕様
サイズ：96T
測定タイプ：Sandwich
検出方法：Colormetric
感度：0.13ng/mL
測定範囲：0.14~100ng/mL
測定原理：This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided inthis kit has been pre-coated with an antibody specific to Human TF. Samples (orStandards) and biotinylated detection antibody specific for Human TF are addedto the micro ELISA plate wells. Human TF would combined with the specificantibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are addedsuccessively to each micro plate well and incubated. Free components are washedaway. The substrate solution is added to each well. Only those wells thatcontain Human TF, biotinylated detection antibody and Avidin-HRP conjugate willappear blue in color. The enzyme-substrate reaction is terminated by theaddition of stop solution and the color turns yellow. The optical density (OD)is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD valueis proportional to the concentration of Human TF. You can calculate theconcentration of Human TF in the samples by comparing the OD of the samples tothe standard curve.
