測定原理：This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E3. During the reaction, E3 in the sample or standard competes with a fixed amount of E3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to E3. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase(HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of E3 in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
測定タイプ：Competitive
検出方法：Colormetric
感度：3.70pg/mL
測定範囲：7.81~500pg/mL
測定原理：This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E3. During the reaction, E3 in the sample or standard competes with a fixed amount of E3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to E3. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase(HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of E3 in the samples is then determined by comparing the OD of the samples to the standard curve.
