89-7830-71 ［取扱停止］Recombinant Rat CD73/NT5E Protein (His Tag)(Active) 10ug PKSR030221
[Elabscience]
特徴
- 背景：5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
仕様
- サイズ：10ug
- 種由来：Rat
- 発現宿主：HEK293 Cells
- 純度：> 90 % as determined by SDS-PAGE
- エンドトキシン濃度：< 1.0 EU per μg of the protein as determined by the LAL method
- アクセッションＮｏ．：Q66HL0
- タグ種類：C-His
- アミノ酸配列：Met1-Lys549
- 遺伝子構造：A DNA sequence encoding the rat NT5E(Q66HL0) (Met1-Lys549) was expressed with a polyhistidine tag at the C-terminus.
- 活性：Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (R&D Systems, Catalog # DY996).The specific activity is >15,000 pmol/min/ug.
- 別名（遺伝子名）：CD73; E5NT;Nucleotidase; NT5E;ecto-5'-nucleotidase;5'-Nucleotidase
- 分子量：59.4 kDa
- 組成：Lyophilized from sterile PBS, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-38352-Elabscience-PKSR030221.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7830-72
Recombinant Rat CD73/NT5E Protein (His Tag)(Active) 20ug
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89-7830-71
Recombinant Rat CD73/NT5E Protein (His Tag)(Active) 10ug
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