89-7818-45 ［取扱停止］Recombinant Mouse BCL2L1/Bcl-XL Protein (aa 1-212, His Tag)(Active) 20ug PKSM040918
[Elabscience]
特徴
- 背景：B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1) , belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.
仕様
- サイズ：20ug
- 種由来：Mouse
- 発現宿主：E.coli
- 純度：> 95 % as determined by SDS-PAGE
- エンドトキシン濃度：Please contact us for more information.
- アクセッションＮｏ．：Q64373-1
- タグ種類：C-His
- アミノ酸配列：Met 1-Arg 212
- 遺伝子構造：A DNA sequence encoding the mouse BCL2L1 (Q64373-1) (Met 1-Arg 212) was expressed, with a C-terminal polyhistidine tag.
- 活性：1. Measured by its binding ability in a functional ELISA.2. Immobilized human BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL.3. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 7.1 ng/mL.
- 別名（遺伝子名）：Bcl(X)L;bcl-x;Bcl-XL;bcl2-L-1;Bcl2l;BclX
- 分子量：25.2 kDa
- 組成：Lyophilized from sterile PBS, pH 7.5
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-107215-Elabscience-PKSM040918.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7818-46
Recombinant Mouse BCL2L1/Bcl-XL Protein (aa 1-212, His Tag)(Active) 100ug
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89-7818-45
Recombinant Mouse BCL2L1/Bcl-XL Protein (aa 1-212, His Tag)(Active) 20ug
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