89-7818-39 ［取扱停止］Recombinant Mouse Gremlin 1/GREM1 Protein (His Tag)(Active) 50ug PKSM040914
[Elabscience]
特徴
- 背景：GREM1 belongs to the DAN family. It contains 1 CTCK (C-terminal cystine knot-like) domain. GREM1 is a cysteine knot-secreted protein and acts as an inhibitor in the TGF beta signaling pathway. It inhibits BMP-2, -4, and -7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development. It interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. As a cytokine, GREM1 may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as inhibitor of monocyte chemotaxis. GREM1 is highly expressed in small intestine, fetal brain and colon.
仕様
- サイズ：50ug
- 種由来：Mouse
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 97 % as determined by SDS-PAGE
- エンドトキシン濃度：< 1.0 EU per μg of the protein as determined by the LAL method.
- アクセッションＮｏ．：O70326
- タグ種類：C-His
- アミノ酸配列：Met1-Asp184
- 遺伝子構造：A DNA sequence encoding the mouse GREM1 (O70326) (Met1-Asp184) was fused with a polyhistidine tag at the C-terminus.
- 活性：Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 1-7 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
- 別名（遺伝子名）：Cktsf1b1;Drm;Grem;ld
- 分子量：19.7 kDa
- 組成：Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 0.5mM EDTA, 3mM DTT
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-107213-Elabscience-PKSM040914.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7818-40
Recombinant Mouse Gremlin 1/GREM1 Protein (His Tag)(Active) 100ug
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89-7818-39
Recombinant Mouse Gremlin 1/GREM1 Protein (His Tag)(Active) 50ug
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