89-7811-63 ［取扱停止］Recombinant Mouse Motch A/NOTCH1 Protein (His Tag)(Active) 100ug PKSM040558
[Elabscience]
特徴
- 背景：NOTCH1 is one of the four mammalian Notch receptors, which is involved in the Notch signaling pathway. Specifically, NOTCH1 promotes the proliferation of myogenic precursor cells, and the NICD domain of NOTCH1 can impair regeneration of skeletal muscles.NOTCH1 is a prevalent signaling pathway in T cell acute lymphoblastic leukemia (T-ALL).The NOTCH signaling pathway is a conserved signaling cascade that regulates many aspects of development and homeostasis in multiple organ systems.The proto-oncogene NOTCH1 is frequently mutated in around 1% of patients with chronic lymphocytic leukemia (CLL). NOTCH1 mutations in oral squamous cell carcinoma (OSCC) frequently occur near the ligand-binding region. These mutations change the domain structure of this protein and affect the ligand binding activity. When NOTCH1 is activated by ligand binding, NOTCH1 intracellular domain (NICD) is cleaved from the cell membrane.
仕様
- サイズ：100ug
- 種由来：Mouse
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 82 % as determined by SDS-PAGE
- エンドトキシン濃度：< 1.0 EU per μg of the protein as determined by the LAL method.
- アクセッションＮｏ．：NP_032740.3
- タグ種類：C-His
- アミノ酸配列：Met 1-Gln 526
- 遺伝子構造：A DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed，with a C-terminal polyhistidine tag.
- 活性：1. Measured by its binding ability in a functional ELISA.2. Immobilized human DLL4 at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL.3. Immobilized mouse DLL4-his at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL.
- 別名（遺伝子名）：Neurogenic locus notch homolog protein 1; Notch 1; Motch A; Mt14;lin-12;Mis6;N1;Tan1
- 分子量：55 kDa
- 組成：Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-107111-Elabscience-PKSM040558.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7811-62
Recombinant Mouse Motch A/NOTCH1 Protein (His Tag)(Active) 50ug
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89-7811-63
Recombinant Mouse Motch A/NOTCH1 Protein (His Tag)(Active) 100ug
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