89-7800-35 Recombinant Human FGF-2/FGF basic/FGFb Protein (aa 1-155, Lus128Asn) 10ug PKSH033503
[Elabscience]
特徴
- 背景：Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro; including mitogenesis; cellular migration; differentiation; angiogenesis; and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media; without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable; daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule; so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies; and maintained cell growth; pluripotency and differentiation potential with a 2-day feeding schedule.
仕様
- サイズ：10ug
- 種由来：Human
- 発現宿主：E.coli
- 純度：> 95% as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per μg as determined by the LAL method.
- アクセッションＮｏ．：P09038-2
- アミノ酸配列：Met1-Ser155(Lus128Asn)
- 遺伝子構造：Recombinant Human FGF-2(K128N) is produced by our E.coli expression system and the target gene encoding Met1-Ser155(Lus128Asn) is expressed.
- 別名（遺伝子名）：Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB
- 分子量：17.2 kDa
- 組成：Lyophilized from a 0.2 μm filtered solution of 20mM Tris;200mM NaCl; pH7.5.
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-229336-Elabscience-PKSH033503.pdf
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| 商品イメージ | アズワン品番 商品名 |
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89-7800-35
Recombinant Human FGF-2/FGF basic/FGFb Protein (aa 1-155, Lus128Asn) 10ug
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89-7800-36
Recombinant Human FGF-2/FGF basic/FGFb Protein (aa 1-155, Lus128Asn) 50ug
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