89-7793-99 ［取扱停止］Recombinant Human TNF-alpha/TNFA Protein High Active Mutant Protein 50ug PKSH033167
[Elabscience]
特徴
- 背景：Tumor Necrosis Factor-α (TNF-α) is secreted by macrophages; monocytes; neutrophils; T-cells; and NK-cells following stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-α while cells that express CD8 secrete little or no TNF-α. Synthesis of TNF-α can be induced by many different stimuli including interferons; IL2; and GM-CSF. The clinical use of the potent anti-tumor activity of TNF-α has been limited by the proinflammatory side effects such as fever; dose-limiting hypotension; hepatotoxicity; intravascular thrombosis; and hemorrhage. Designing clinically applicable TNF-α mutants with low systemic toxicity has been of intense pharmacological interest. Human TNF-α that binds to murine TNF-R55 but not murine TNF-R7; exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-α; which binds to both murine TNF receptors. Based on these results; many TNF-α mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro and have exhibited lower systemic toxicity in vivo. Recombinant Human TNF-α High Active Mutant differs from the wild-type by amino acid subsitution of amino acids 1-7 with Arg8; Lys9; Arg10 and Phe157. This mutant form has been shown to have increased activity with less inflammatory side effects in vivo.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：E.coli
- 純度：> 95 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：P01375
- 遺伝子構造：Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding is expressed.
- 活性：Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 1.48 pg/ml.
- 別名（遺伝子名）：Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
- 分子量：16.9 kDa
- 組成：Lyophilized from a 0.2 μm filtered solution of 20mM PB; 150mM NaCl; pH 7.4.
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-106837-Elabscience-PKSH033167.pdf
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89-7793-98
Recombinant Human TNF-alpha/TNFA Protein High Active Mutant Protein 10ug
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89-7793-99
Recombinant Human TNF-alpha/TNFA Protein High Active Mutant Protein 50ug
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