特徴
- 背景：Malate Dehydrogenase, Cytoplasmic (MDH1) is an enzyme which belongs to the MDH Type 2 sub-family of LDH/MDH superfamily. MDH1 is involved in the Citric Acid Cycle that catalyzes the conversion of Malate into Oxaloacetate (using NAD+) and vice versa. MDH1 should not be confused with Malic Enzyme, which catalyzes the conversion of Malate to Pyruvate, producing NADPH. MDH1 also participates in Gluconeogenesis, the synthesis of Glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by Pyruvate Carboxylase to form Pxaloacetate, a Citric Acid Cycle intermediate. In order to transport the Oxaloacetate out of the Mitochondria, Malate Dehydrogenase reduces it to Malate, and it then traverses the inner Mitochondrial membrane. Once in the cytosol, the Malate is oxidized back to Oxaloacetate by MDH1. Finally, Phosphoenol-Pyruvate Carboxy Kinase (PEPCK) converts Oxaloacetate to Phosphoenol Pyruvate.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：E.coli
- 純度：> 95 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：P40925
- タグ種類：C-6His
- アミノ酸配列：Ser2-Ala334
- 遺伝子構造：Recombinant Human Malate Dehydrogenase, Cytoplasmic is produced by our E.coli expression system and the target gene encoding Ser2-Ala334 is expressed with a 6His tag at the C-terminus.
- 別名（遺伝子名）：Malate Dehydrogenase Cytoplasmic; Cytosolic Malate Dehydrogenase; Diiodophenylpyruvate Reductase; MDH1; MDHA
- 分子量：37.5 kDa
- 組成：Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
- 保存条件：Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-106442-Elabscience-PKSH032730.pdf
- この商品は法規制を確認しておりません。（法規制によって販売できない場合もございます）
- 【試薬に関するお問合せ】
- アズワン株式会社 試薬・プロセス材料グループ
- TEL：06-6447-8641
- FAX：06-6447-8642
- E-mail：[email protected]
商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
|
89-7786-09
Recombinant Human MDH1 Protein (His Tag) 10ug
|
|
|
89-7786-10
Recombinant Human MDH1 Protein (His Tag) 50ug
|
よくあるご質問（FAQ）
掲載カタログ情報
| 掲載カタログ名 | 掲載ページ |
|---|