特徴
- 背景：Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family. The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB).
仕様
- サイズ：10ug
- 種由来：Human
- 発現宿主：Human Cells
- 純度：> 95 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：Q15046
- タグ種類：C-6His
- アミノ酸配列：Ala2-Val597
- 遺伝子構造：Recombinant Human Lysine--tRNA Ligase is produced by our Mammalian expression system and the target gene encoding Ala2-Val597 is expressed with a 6His tag at the C-terminus.
- 別名（遺伝子名）：Lysine--tRNA Ligase; Lysyl-tRNA Synthetase; LysRS; KARS; KIAA0070
- 分子量：69.1 kDa
- 組成：Supplied as a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,1mM DTT,20% glycerol,pH8.0.
- 保存条件：Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-106433-Elabscience-PKSH032720.pdf
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- アズワン株式会社 試薬・プロセス材料グループ
- TEL：06-6447-8641
- FAX：06-6447-8642
- E-mail：[email protected]
商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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89-7785-93
Recombinant Human KARS Protein (His Tag) 50ug
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89-7785-92
Recombinant Human KARS Protein (His Tag) 10ug
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