特徴
- 背景：As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity.
仕様
- サイズ：10ug
- 種由来：Human
- 発現宿主：Human Cells
- 純度：> 95 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：P01857
- アミノ酸配列：Asp104-Lys330
- 遺伝子構造：Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330 is expressed.
- 活性：Immobilized Human IgG1 Fc at 2μg/ml(100 μl/well) can bind Human CD64-His(Cat: PKSH033655). The ED50 of Human IgG1 Fc is 47.99 ng/ml .
- 別名（遺伝子名）：g gamma-1 chain C region;IGHG1
- 分子量：41.6 kDa
- 組成：Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-106286-Elabscience-PKSH032558.pdf
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- E-mail：[email protected]
商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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89-7782-98
Recombinant Human IgG1 Fc Protein (Active) 50ug
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89-7782-97
Recombinant Human IgG1 Fc Protein (Active) 10ug
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