特徴
- 背景：Matrix Metalloproteinase-2 (MMP-2) is an enzyme that degrades components of the extracellular matrix and thus plays a pivotal role in cell migration during physiological and pathological processes. MMP-2 expression is dependent on extracellular matrix metalloproteinase inducer (EMMPRIN); Her2/neu; growth factors; cytokines; and hormones. Pro-MMP-2 activation needs MT1-MMP and TIMP-2 contribution. MMP-2 is changed in distribution and increased in amount in the ventral cochlear nucleus after unilateral cochlear ablation. A low level of MMP-2 is linked to favorable prognosis in patients with a hormone receptor-negative tumor; usually associated with high risk. As a zymogen requiring proteolytic activation for catalytic activity; MMP-2 has been implicated broadly in the invasion and metastasis of many cancer model systems; including human breast cancer (HBC). Blocking MMP-2 secretion and activation during breast carcinoma development may decrease metastasis. The detection of active MMP-2 alone or the rate of pro-MMP-2 and active MMP-2 is considered a very sensitive indicator of cancer metastasis. Modulation of MMP-2 expression and activation through specific inhibitors and activators may thus provide a new mechanism for breast cancer treatment.
仕様
- サイズ：20ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> (74.7+17.3) % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug of the protein as determined by the LAL method.
- アクセッションＮｏ．：NP_004521.1
- アミノ酸配列：Met 1-Cys 660
- 遺伝子構造：A DNA sequence encoding the native human MMP2 (NP_004521.1) (Met 1-Cys 660) was expressed and purified.
- 活性：Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (AnaSpec; Catalog # 27076). The specific activity is > 1;000 pmoles/min/ug.2. Measured by its binding ability in a functional ELISA.3. Immobilized human MMP2 at 10 μg/mL (100 μl/well) can bind human TIMP2/Fc. The EC50 of human TIMP2/Fc is 0.02 μg/mL.(Activation description: The proenzyme needs to be activated by APMA for an activated form)
- 別名（遺伝子名）：72 kDa Type IV Collagenase; 72 kDa Gelatinase; Gelatinase A; Matrix Metalloproteinase-2; MMP-2; TBE-1; MMP2; CLG4A;CLG4;MMP-II;MONA;TBE-1
- 分子量：
- 組成：Lyophilized from sterile PBS; pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-38257-Elabscience-PKSH031888.pdf
- この商品は法規制を確認しておりません。（法規制によって販売できない場合もございます）
- 【試薬に関するお問合せ】
- アズワン株式会社 試薬・プロセス材料グループ
- TEL：06-6447-8641
- FAX：06-6447-8642
- E-mail：[email protected]
商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
|
89-7771-67
Recombinant Human MMP-2 Protein (Active) 20ug
|
|
|
89-7771-66
Recombinant Human MMP-2 Protein (Active) 10ug
|
よくあるご質問（FAQ）
掲載カタログ情報
| 掲載カタログ名 | 掲載ページ |
|---|