特徴
- 背景：Vaccinia H1-related phosphatase (VHR) is classified as a dual-specificity phosphatase (DUSP); and the other name is dual-specificity phosphatase 3 (DUSP3). DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. Unlike typical DUSPs; VHR lacks mitogen-activated protein kinase (MAPK)-binding domain; and shows poor activity against MAPKs. VHR often act on bisphosphorylated protein substrates; it displays a strong preference for dephosphorylating phosphotyrosine residues over phosphothreonine residues. VHR has been identified as a novel regulator of extracellular regulated kinases (ERKs). VHR is responsible for the rapid inactivation of ERK following stimulation and for its repression in quiescent cells. VHR is a negative regulator of the Erk and Jnk pathways in T cells and; therefore; may play a role in aspects of T lymphocyte physiology that depend on these kinases.
仕様
- サイズ：100ug
- 種由来：Human
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 95 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：P51452
- アミノ酸配列：Met 1-Pro 185
- 遺伝子構造：A DNA sequence encoding the human VHR (P51452)(Met 1-Pro 185)was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
- 活性：Measured by hydrolysis of 250 uM 3-O-methyl fluorescein phosphate (OMFP).The specific activity is >1.0 umol/min/m
- 別名（遺伝子名）：Dual specificity protein phosphatase 3;DUSP3;Dual specificity protein phosphatase VHR;Vaccinia H1-related phosphatase;VHR
- 分子量：20.6 kDa
- 組成：Lyophilized from sterile 20mM Tris; 500mM NaCl; 10% glycerol; pH 8.0
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-38238-Elabscience-PKSH031861.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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89-7771-18
Recombinant Human DUSP3/VHR Protein (Active) 50ug
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89-7771-19
Recombinant Human DUSP3/VHR Protein (Active) 100ug
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