89-7769-37 ［取扱停止］Recombinant Human E-Cadherin/CDH1 Protein (His Tag)(Active) 200ug PKSH031763
[Elabscience]
特徴
- 背景：Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
仕様
- サイズ：200ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 95 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug of the protein as determined by the LAL method.
- アクセッションＮｏ．：NP_004351.1
- タグ種類：C-His
- アミノ酸配列：Met 1-Ile 707
- 遺伝子構造：A DNA sequence encoding the extracellular domain of human E-Cad precusor (NP_004351.1) (Met 1-Ile 707) was fused with a C-terminal polyhistidine tag.
- 活性：Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells.When cells are added to E-Cad coated plates (5 μg/mL, 100 μL/well), approximately 20%-40% of cells will adhere specifically after 90 minutes at 37 ℃.
- 別名（遺伝子名）：Cadherin-1; CDH1;CAM 120/80;E-cadherin; CD324;CDHE;E-cad;E-Cadherin;ECAD;LCAM;UVO;Arc-1
- 分子量：77 kDa
- 組成：Lyophilized from sterile PBS, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-105720-Elabscience-PKSH031763.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7769-37
Recombinant Human E-Cadherin/CDH1 Protein (His Tag)(Active) 200ug
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89-7769-36
Recombinant Human E-Cadherin/CDH1 Protein (His Tag)(Active) 100ug
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