89-7769-27 Recombinant Human DDR2 Kinase/CD167b Protein (Fc Tag)(Active) 100ug PKSH031758
[Elabscience]
特徴
- 背景：Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
仕様
- サイズ：100ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 95 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_001014796.1
- タグ種類：C-Fc
- アミノ酸配列：Met 1-Arg 399
- 遺伝子構造：A DNA sequence encoding the extracellular domain (Met 1-Arg 399) of human DDR2 precursor (NP_001014796.1) was expressed with the fused Fc region of human IgG1 at the C-terminus.
- 活性：Measured by its binding ability in a functional ELISA. Immobilized Rat tail Collagen I at 10 μg/ml can bind recombinant human DDR2-Fc Chimera with a linear range of 2.5-80 ng/ml. Scatchard analysis showed the affinity constant (Kd) of recombinant human DDR2-Fc Chimera bound to rat tail collagen I was 6.8 nM.
- 別名（遺伝子名）：CD167;MIG20a;NTRKR3;TKT;TYRO10
- 分子量：69.4 kDa
- 組成：Lyophilized from sterile PBS, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-38155-Elabscience-PKSH031758.pdf
- この商品は法規制を確認しておりません。（法規制によって販売できない場合もございます）
- 【試薬に関するお問合せ】
- アズワン株式会社 試薬・プロセス材料グループ
- TEL：06-6447-8641
- FAX：06-6447-8642
- E-mail：[email protected]
商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
|
89-7769-27
Recombinant Human DDR2 Kinase/CD167b Protein (Fc Tag)(Active) 100ug
|
|
|
89-7769-26
Recombinant Human DDR2 Kinase/CD167b Protein (Fc Tag)(Active) 50ug
|
よくあるご質問（FAQ）
掲載カタログ情報
| 掲載カタログ名 | 掲載ページ |
|---|