89-7768-55 ［取扱停止］Recombinant Human Noggin/NOG Protein (His Tag)(Active) 20ug PKSH031720
[Elabscience]
特徴
- 背景：Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities; with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs; Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7; and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes; including embryonic dorsal-ventral axis; induction of neural tissue; formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4; along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development; joint formation; and neural tube fusion.
仕様
- サイズ：20ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 95 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_005441.1
- タグ種類：C-His
- アミノ酸配列：Met 1-Cys 232
- 遺伝子構造：A DNA sequence encoding the human Noggin precursor (NP_005441.1) (Met 1-Cys 232) was fused with a polyhistidine tag at the C-terminus.
- 活性：Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 0.05-0.3 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
- 別名（遺伝子名）：Noggin;SYM1;SYNS1
- 分子量：24.6 kDa
- 組成：Lyophilized from sterile PBS; pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-105708-Elabscience-PKSH031720.pdf
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| 商品イメージ | アズワン品番 商品名 |
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89-7768-56
Recombinant Human Noggin/NOG Protein (His Tag)(Active) 100ug
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89-7768-55
Recombinant Human Noggin/NOG Protein (His Tag)(Active) 20ug
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