89-7767-79 ［取扱停止］Recombinant Human GFRA1/GDNFRA Protein (aa 1-424, His Tag)(Active) 200ug PKSH031680
[Elabscience]
特徴
- 背景：Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-β (TGF-â) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.
仕様
- サイズ：200ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 98 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_665736.1
- タグ種類：C-His
- アミノ酸配列：Met 1-Ser 424
- 遺伝子構造：A DNA sequence encoding the human GFRα1 without the propeptide (NP_665736.1) (Met 1-Ser 424) was fused with the a polyhistidine tag at the C-terminus.
- 活性：1. Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.2-1 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.2. Measured by its ability to bind human GDNF (native) in functional ELISA.
- 別名（遺伝子名）：GDNF Family Receptor Alpha-1; GDNF Receptor Alpha-1; GDNFR-Alpha-1; GFR-Alpha-1; RET Ligand 1; TGF-Beta-Related Neurotrophic Factor Receptor 1; GFRA1; GDNFRA; RETL1; TRNR1
- 分子量：46 kDa
- 組成：Lyophilized from sterile PBS, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-38099-Elabscience-PKSH031680.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7767-78
Recombinant Human GFRA1/GDNFRA Protein (aa 1-424, His Tag)(Active) 100ug
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89-7767-79
Recombinant Human GFRA1/GDNFRA Protein (aa 1-424, His Tag)(Active) 200ug
|
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