89-7767-71 ［取扱停止］Recombinant Human CD50/ICAM-3 Protein (His Tag)(Active) 50ug PKSH031675
[Elabscience]
特徴
- 背景：The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes, and is thus an important molecule for the first immune response. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase of cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 92 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_002153.2
- タグ種類：C-His
- アミノ酸配列：Met 1-His 485
- 遺伝子構造：A DNA sequence encoding the extracellular domain of human ICAM3 (NP_002153.2) (Met 1-His 485) was expressed, with the C-terminal fused polyhistidine tag.
- 活性：Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When cells are added to ICAM3-coated plates (12.5 μg/mL , 100 μL/well), approximately >25% cells will adhere specifically.
- 別名（遺伝子名）：CD50;CDW50;ICAM-3;ICAM-R
- 分子量：50.8 kDa
- 組成：Lyophilized from sterile PBS, pH 7.5
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-38095-Elabscience-PKSH031675.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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89-7767-70
Recombinant Human CD50/ICAM-3 Protein (His Tag)(Active) 20ug
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89-7767-71
Recombinant Human CD50/ICAM-3 Protein (His Tag)(Active) 50ug
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