89-7767-45 ［取扱停止］Recombinant Human ICAM-1/CD54 Protein (His & Fc Tag)(Active) 100ug PKSH031662
[Elabscience]
特徴
- 背景：Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.
仕様
- サイズ：100ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 95 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_000192.2
- タグ種類：C-His & Fc
- アミノ酸配列：Met 1-Glu 480
- 遺伝子構造：A DNA sequence encoding the human ICAM1 (NP_000192.2) extracellular domain (Met 1-Glu 480) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
- 活性：Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When 5 x 10E4 cells/well are added to Recombinant Human ICAM-1/CD54 coated plates (12.5 μg/mL with 100 μL/well), >40% cells will adhere after 1 hour of incubation at 37℃.
- 別名（遺伝子名）：BB2;CD54;ICAM-1;P3.58
- 分子量：77.4 kDa
- 組成：Lyophilized from sterile PBS, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-38089-Elabscience-PKSH031662.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7767-45
Recombinant Human ICAM-1/CD54 Protein (His & Fc Tag)(Active) 100ug
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89-7767-44
Recombinant Human ICAM-1/CD54 Protein (His & Fc Tag)(Active) 50ug
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