89-7767-13 Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag)(Active) 20ug PKSH031645
[Elabscience]
特徴
- 背景：Sonic HedgeHog; also known as sonic hedgehog protein; belongs to the hedgehog family. It cannot be detected in adult tissues while can be found in fetal intestine; liver; lung; and kidney. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube; the anterior-posterior limb axis; and the ventral somites. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites; and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog binds to the patched receptor; which functions in association with smoothened; to activate the transcription of target genes. In the absence of sonic HedgeHog; patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor; the gli oncogene. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5; triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type 3.
仕様
- サイズ：20ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 95 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：Q15465
- タグ種類：C-His
- アミノ酸配列：Met 1-Gly 197
- 遺伝子構造：A DNA sequence encoding the amino acid sequence (Met 1-Gly 197) of human SHH (Q15465); that is Sonic hedgehog protein N-product; was fused with a polyhistidine tag at the C-terminus.
- 活性：Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura; T. et al. (1997) Biochem. Biophys. Res. Commun.237:465.The ED50 for this effect is typically 2-10 μg/mL.
- 別名（遺伝子名）：Sonic Hedgehog Protein; SHH; HHG-1; SHH
- 分子量：21 kDa
- 組成：Lyophilized from sterile PBS; pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-38076-Elabscience-PKSH031645.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7767-12
Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag)(Active) 5ug
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89-7767-13
Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag)(Active) 20ug
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