89-7767-10 ［取扱停止］Recombinant Human CD32a/FCGR2A Protein (166 Arg, His Tag)(Active) 50ug PKSH031644
[Elabscience]
特徴
- 背景：Interferon-alpha/beta receptor beta chain (IFNAR2) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Initial cell-surface IFNAR2 expression at diagnosis assessed by flow cytometry widely distributed but showed overall significantly higher expression in CML patients when compared with normal controls. In 15 fresh patients who subsequently received IFNα therapy, IFNAR2 expression at diagnosis was significantly higher in cytogenetic good responders than in poor responders. Down-regulation of IFNAR2 expression during IFNα therapy was observed only in good responders but not in poor responders. The encoded protein also functions as an antiviral factor. IFNAR2 may associate with IFNAR1 to form the type I interferon receptor. This protein serves as a receptor for interferons alpha and beta. IFNAR2 is also involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity. Following binding of IFNα2, IFNAR2 is internalized, but, instead of being routed towards degradation as it is when complexed to IFNβ, it recycles back to the cell surface.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 90 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug of the protein as determined by the LAL method.
- アクセッションＮｏ．：AAH19931.1
- タグ種類：C-His
- アミノ酸配列：Met 1-Gly 216
- 遺伝子構造：A DNA sequence encoding the extracellular domain (Met 1-Gly 216) of human CD32a (AAH19931.1) precursor was expressed, with a C-terminal polyhistidine tag.
- 活性：Measured by its binding ability in a functional ELISA. Immobilized human CD32a at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.07-0.17 μg/ml.
- 別名（遺伝子名）：Low affinity immunoglobulin gamma Fc region receptor II-a; IgG Fc receptor II-a; CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD32; FCGR2A; FCG2; FCGR2A1;IGFR2;CD32A;CDw32;Fc gamma RIIA;FCG2;FcGR;FCGR6
- 分子量：22 kDa
- 組成：Lyophilized from sterile 20mM Tris, 50mM NaCl, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-38075-Elabscience-PKSH031644.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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89-7767-10
Recombinant Human CD32a/FCGR2A Protein (166 Arg, His Tag)(Active) 50ug
|
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89-7767-11
Recombinant Human CD32a/FCGR2A Protein (166 Arg, His Tag)(Active) 100ug
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