89-7766-13 ［取扱停止］Recombinant Human TRAIL R2/TNFRSF10B Protein (His & Fc Tag)(Active) 100ug PKSH031592
[Elabscience]
特徴
- 背景：Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
仕様
- サイズ：100ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 95 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_003833.3
- タグ種類：C-His & Fc
- アミノ酸配列：Met 1-Glu 182
- 遺伝子構造：A DNA sequence encoding the human TNFRSF10B (NP_003833.3) extracellular domain (Met 1-Glu 182) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
- 活性：1. Measured by its binding ability in a functional ELISA. Immobilized human TNFSF10 at 10 μg/ml (100 μl/well) can bind human TNFRSF10B Fc chimera with a linear range of 2.5-40 ng/ml.2. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 25-125 ng/mL in the presence of 10 ng/mL Recombinant Human TRAIL/TNFSF10.
- 別名（遺伝子名）：Tumor Necrosis Factor Receptor Superfamily Member 10B; Death Receptor 5; TNF-Related Apoptosis-Inducing Ligand Receptor 2; TRAIL Receptor 2; TRAIL-R2; CD262; TNFRSF10B; DR5; KILLER; TRAILR2; TRICK2; ZTNFR9
- 分子量：42.4 kDa
- 組成：Lyophilized from sterile PBS, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-105675-Elabscience-PKSH031592.pdf
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| 商品イメージ | アズワン品番 商品名 |
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89-7766-13
Recombinant Human TRAIL R2/TNFRSF10B Protein (His & Fc Tag)(Active) 100ug
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89-7766-14
Recombinant Human TRAIL R2/TNFRSF10B Protein (His & Fc Tag)(Active) 200ug
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