特徴
- 背景：The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family; which contains only the BH3 domain; and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule; it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release; which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form; tBid; to facilitate the release of apoptogenic proteins like cytochrome c.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：E.coli
- 純度：> 90 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：Please contact us for more information.
- アクセッションＮｏ．：P55957-1
- アミノ酸配列：Met 1-Asp 195
- 遺伝子構造：A DNA sequence encoding the human BID isoform 1 (P55957-1) (Met 1-Asp 195) was expressed and purified; with additional two amino acids (Gly & Pro) at the N-terminus.
- 活性：1. Measured by its binding ability in a functional ELISA.2. Immobilized human BID at 10 μg/mL (100 μl/well) can bind biotinylated human BCL2L1; The EC50 of biotinylated human BCL2L1 is 7.1 ng/mL.3. Immobilized human BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1; The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL.
- 別名（遺伝子名）：BH3-Interacting Domain Death Agonist; p22 BID; BID
- 分子量：
- 組成：Lyophilized from sterile 40mM Tris; 150mM NaCl; pH 8.0
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-38033-Elabscience-PKSH031587.pdf
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- アズワン株式会社 試薬・プロセス材料グループ
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- E-mail：[email protected]
商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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89-7766-07
Recombinant Human BID Protein (Active) 50ug
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89-7766-08
Recombinant Human BID Protein (Active) 100ug
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