89-7765-72 ［取扱停止］Recombinant Human Carboxypeptidase A2/CPA2 Protein (His Tag)(Active) 10ug PKSH031569
[Elabscience]
特徴
- 背景：Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
仕様
- サイズ：10ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 90 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_001860.2
- タグ種類：C-His
- アミノ酸配列：Met 1-Tyr 417
- 遺伝子構造：A DNA sequence encoding the pre pro form of human CPA2 (NP_001860.2) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.
- 活性：Measured by its ability to cleave a colorimetric peptide substrate, N-acetyl-Phe-Thiaphe-OH (N-Ac-PSP, Peptide International's Catalog# STP-3621-PI), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405 nm and the extinction coefficient of 13,260 M-1 cm-The specific activity is >4,000 pmoles/min/μg.
- 別名（遺伝子名）：Carboxypeptidase A2; CPA2
- 分子量：46 kDa
- 組成：Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-38018-Elabscience-PKSH031569.pdf
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| 商品イメージ | アズワン品番 商品名 |
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89-7765-72
Recombinant Human Carboxypeptidase A2/CPA2 Protein (His Tag)(Active) 10ug
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89-7765-73
Recombinant Human Carboxypeptidase A2/CPA2 Protein (His Tag)(Active) 20ug
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