89-7764-35 Recombinant Human TH/Tyrosine Hydroxylase Protein (His Tag) 50ug PKSH031490
[Elabscience]
特徴
- 背景：Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 94 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：P07101-3
- タグ種類：N-His
- アミノ酸配列：Pro 2-Gly 497
- 遺伝子構造：A DNA sequence encoding the human TH isoform 2 (P07101-3) (Pro 2-Gly 497) was fused with a polyhistidine tag at the N-terminus.
- 別名（遺伝子名）：DYT14;DYT5b;TYH
- 分子量：57.6 kDa
- 組成：Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37967-Elabscience-PKSH031490.pdf
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89-7764-35
Recombinant Human TH/Tyrosine Hydroxylase Protein (His Tag) 50ug
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89-7764-34
Recombinant Human TH/Tyrosine Hydroxylase Protein (His Tag) 20ug
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