89-7762-46 ［取扱停止］Recombinant Human Nectin-3/PVRL3 Protein (His Tag)(Active) 50ug PKSH031388
[Elabscience]
特徴
- 背景：Poliovirus receptor-related 3 (PVRL3), also known as Nectin-3 and CD113, is a member of the nectin family. PVRL3/Nectin-3 is an 83 kDa, type I transmembrane glycoprotein. Its precursor is 549 amino acids (aa) in length and contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa, and a cytoplasmic region of 124 aa. Nectin-3 has three splicing variants, nectin-3alpha (biggest), -3beta (middle), and -3gamma (smallest). It is predominantly expressed in testis and placenta as well as in various cell lines, including epithelial cell lines. PVRL3/Nectin-3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/Nectin-2 at Sertoli-spermatid junctions. PVRL3/Nectin-3 is thus involved in the formation of cell-cell junctions, including adherens junctions and synapses. It has been shown to induce endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 98 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_056295.1
- タグ種類：C-His
- アミノ酸配列：Met 1-Asp 400
- 遺伝子構造：A DNA sequence encoding the extracellular domain of human PVRL3 (NP_056295.1) (Met 1-Asp 400) was expressed, with a polyhistidine tag at the C-terminus.
- 活性：Measured by its binding ability in a functional ELISA.Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated Nectin-1 with a linear range of 6.4-800 ng/ml.2. Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind Nectin-1/ Fc chimera with a linear range of 0.156-5 ng/ml.
- 別名（遺伝子名）：Poliovirus Receptor-Related Protein 3; CDw113; Nectin-3; CD113; PVRL3; PRR3;PVRR3
- 分子量：39.3 kDa
- 組成：Lyophilized from sterile PBS, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37904-Elabscience-PKSH031388.pdf
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| 商品イメージ | アズワン品番 商品名 |
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89-7762-46
Recombinant Human Nectin-3/PVRL3 Protein (His Tag)(Active) 50ug
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89-7762-47
Recombinant Human Nectin-3/PVRL3 Protein (His Tag)(Active) 100ug
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