89-7762-25 Recombinant Human MAP1D Protein (His Tag)(Active) 50ug PKSH031376
[Elabscience]
特徴
- 背景：Methionine aminopeptidase 1D, also known as MAP1D, is a member of the peptidase M24A family. N-terminal methionine removal is an important cellular process required for proper biological activity, subcellular localization, and eventual degradation of many proteins. The enzymes that catalyze this reaction are called Methionine aminopeptidases (MAPs). MAP1D is overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). Downregulation of MAP1D expression by shRNA in HCT-116 colon carcinoma cells reduces anchorage-independant growth in soft agar. MAP1D binds two cobalt ions per subunit. The true nature of the physiological cofactor is under debate. MAP1D is also active with zinc, manganese or divalent ions. MAP1D removes the amino-terminal methionine from nascent proteins. It may also play an important role in colon tumorigenesis.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 92 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_954697.1
- タグ種類：C-His
- アミノ酸配列：Arg 44-Ala 335
- 遺伝子構造：A DNA sequence encoding the human MAP1D (NP_954697.1) (Arg 44-Ala 335) was expressed, with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus.
- 活性：Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC, R&D Systems, Catalog#ES017. The resulting GP-AMC is cleaved by human DPPIV/CD26, R&D Systems, Catalog#1180SE. The specific activity is >30 pmoles/min/μg.
- 別名（遺伝子名）：Methionine Aminopeptidase 1D Mitochondrial; Methionyl Aminopeptidase Type 1D Mitochondrial; METAP1D; MAP1D;MAP 1D;MetAP 1D;Metap1l
- 分子量：33.4 kDa
- 組成：Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-105600-Elabscience-PKSH031376.pdf
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| 商品イメージ | アズワン品番 商品名 |
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89-7762-24
Recombinant Human MAP1D Protein (His Tag)(Active) 20ug
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89-7762-25
Recombinant Human MAP1D Protein (His Tag)(Active) 50ug
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