89-7762-06 ［取扱停止］Recombinant Human CCNE1/Cyclin-E1 Protein (His Tag)(Active) 50ug PKSH031365
[Elabscience]
特徴
- 背景：Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 85 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_001229.1
- タグ種類：N-His
- アミノ酸配列：Met 1-Ala 410
- 遺伝子構造：A DNA sequence encoding the full length of human CCNE1 isoform 1 (NP_001229.1) (Met 1-Ala 410) was expressed, with a polyhistidine tag at the N-terminus.
- 活性：Measured by its binding ability in a functional ELISA. Immobilized human CCNE1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK4, The EC50 of biotinylated human CDK4 is 0.10-0.32 μg/ml.
- 別名（遺伝子名）：CCNE
- 分子量：49.3 kDa
- 組成：Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.5, 10% glycerol
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37888-Elabscience-PKSH031365.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7762-05
Recombinant Human CCNE1/Cyclin-E1 Protein (His Tag)(Active) 20ug
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89-7762-06
Recombinant Human CCNE1/Cyclin-E1 Protein (His Tag)(Active) 50ug
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