89-7760-45 ［取扱停止］Recombinant Human PRMT5/SKB1 Protein (His & FLAG Tag) 20ug PKSH031276
[Elabscience]
特徴
- 背景：Methylation of arginine residues is a widespread post-translational modification of proteins catalyzed by a small family of PRMTs. The modification appears to regulate protein functions and interactions that affect gene regulation; signalling and subcellular localization of proteins and nucleic acids. Protein arginine methyltransferase 5 (PRMT5) is a member of the protein arginine N-methyltransferases (PRMT)family; and exists as at least homodimers and homotetramers; or homooligomers mediated by disulfide bonds and non-covalent association ubiquitously. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); and thus plays a role in the assembly and biogenesis of snRNP core particles. PRMT5 methylates histone H2A and H4 'Arg-3' during germ cell development; as well as histone H3 'Arg-8'; which may repress transcription. PRMT5 also methylates SUPT5H and regulates its transcriptional elongation properties. Additionally; it is also suggested that PRMT5 negatively regulates cyclin E1 promoter activity and cellular proliferation.
仕様
- サイズ：20ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 85 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_006100.2
- タグ種類：C-His & N-FLAG
- アミノ酸配列：Ala 2-Leu 637
- 遺伝子構造：A DNA sequence encoding the human PRMT5 (NP_006100.2) (Ala 2-Leu 637) was expressed; fused with a polyhistidine tag at the C-terminus and a Flag tag at the N-terminus.
- 別名（遺伝子名）：HRMT1L5;IBP72;JBP1;SKB1;SKB1Hs
- 分子量：75 kDa
- 組成：Lyophilized from sterile 50mM Tris; 100mM NaCl; pH 8.0; 0.5mM EDTA; 0.5PMSF; 0.5mM; TCEP; 25% glycerol
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37823-Elabscience-PKSH031276.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7760-45
Recombinant Human PRMT5/SKB1 Protein (His & FLAG Tag) 20ug
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89-7760-46
Recombinant Human PRMT5/SKB1 Protein (His & FLAG Tag) 50ug
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