89-7756-85 ［取扱停止］Recombinant Human CD111/Nectin-1 Protein (Fc Tag)(Active) 50ug PKSH031066
[Elabscience]
特徴
- 背景：Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 98 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_002846.3
- タグ種類：C-Fc
- アミノ酸配列：Met 1-Thr 334
- 遺伝子構造：A DNA sequence encoding the human PVRL1 isoform 1 (NP_002846.3) extracellular domain (Met 1-Thr 334) was was fused with the Fc region of human IgG1 at the C-terminus.
- 活性：Measured by its binding ability in a functional ELISA. Immobilized rh PVRL3 at 1 μg/ml (100 μl/well) can bind recombinant human Nectin-1 with a linear range of 0.156-5 ng/ml.
- 別名（遺伝子名）：Poliovirus Receptor-Related Protein 1; Herpes Virus Entry Mediator C; Herpesvirus Entry Mediator C; HveC; Herpesvirus Ig-Like Receptor; HIgR; Nectin-1; CD111; PVRL1; HVEC; PRR1;ED4;HIgR;HV1S;HVEC;nectin-1;OFC7;PRR;PVRR;PVRR1;SK-12
- 分子量：61 kDa
- 組成：Lyophilized from sterile PBS, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37665-Elabscience-PKSH031066.pdf
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| 商品イメージ | アズワン品番 商品名 |
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89-7756-86
Recombinant Human CD111/Nectin-1 Protein (Fc Tag)(Active) 100ug
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89-7756-85
Recombinant Human CD111/Nectin-1 Protein (Fc Tag)(Active) 50ug
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