89-7756-63 ［取扱停止］Recombinant Human UCHL3/UCH-L3 Protein (His Tag)(Active) 20ug PKSH031053
[Elabscience]
特徴
- 背景：Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.
仕様
- サイズ：20ug
- 種由来：Human
- 発現宿主：E.coli
- 純度：> 97 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：Please contact us for more information.
- アクセッションＮｏ．：NP_005993.1
- タグ種類：N-His
- アミノ酸配列：Glu 2-Ala 230
- 遺伝子構造：A DNA sequence encoding the human UCHL3 (NP_005993.1) (Glu 2-Ala 230) was expressed, with a polyhistide tag at the N-terminus.
- 活性：Measured by the hydrolysis of UbiquitinAMC. The specific activity is >14,000 pmoles/min/μg.
- 別名（遺伝子名）：Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3; UCH-L3; Ubiquitin Thioesterase L3; UCHL3
- 分子量：27 kDa
- 組成：Lyophilized from sterile 20mM Tris, 500mM NaCl, 20% glycerol, 1mM DTT, pH 8.0
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37655-Elabscience-PKSH031053.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7756-64
Recombinant Human UCHL3/UCH-L3 Protein (His Tag)(Active) 50ug
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89-7756-63
Recombinant Human UCHL3/UCH-L3 Protein (His Tag)(Active) 20ug
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