89-7755-52 ［取扱停止］Recombinant Human Kallikrein 4/KLK4 Protein (His Tag)(Active) 10ug PKSH030981
[Elabscience]
特徴
- 背景：Kallikrein-4, also known as Enamel matrix serine proteinase 1, Kallikrein-like protein 1, KLK-L1, Serine protease 17, KLK4, PRSS17 and EMSP1, is a secreted protein which belongs to the peptidase S1 family and Kallikrein subfamily. Kallikrein-4 / KLK4 is a serine protease expressed during enamel maturation, and proteolytic processing of the enamel matrix by KLK4 is critical for proper enamel formation. Kallikrein-4 / KLK4 contains one peptidase S1 domain. Kallikrein-4 / KLK4 is secreted by transition- and maturation-stage ameloblasts. KLK4 aggressively degrades the retained organic matrix following the termination of enamel protein secretion. Two proteases are secreted into the enamel matrix of developing teeth. The early protease is enamelysin (MMP-20). The late protease is kallikrein 4 (KLK4). The principle functions of MMP-20 and KLK4 in dental enamel formation are to facilitate the orderly replacement of organic matrix with mineral, generating an enamel layer that is harder, less porous, and unstained by retained enamel proteins. Defects in Kallikrein-4 / KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) which is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions.
仕様
- サイズ：10ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 94 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_004908.3
- タグ種類：C-His
- アミノ酸配列：Met 1-Ser 254
- 遺伝子構造：A DNA sequence encoding the human KLK4 (NP_004908.3) (Met 1-Ser 254) was expressed, with a polyhistidine tag at the C-terminus.
- 活性：Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. (R&D Systems, Catalog # ES011). The specific activity is >250 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
- 別名（遺伝子名）：Kallikrein-4; Enamel Matrix Serine Proteinase 1; Kallikrein-Like Protein 1; KLK-L1; Prostase; Serine Protease 17; KLK4; EMSP1; PRSS17; PSTS;AI2A1;ARM1;EMSP;kallikrein;KLK-L1
- 分子量：25.8 kDa
- 組成：Lyophilized from sterile PBS, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-105499-Elabscience-PKSH030981.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7755-52
Recombinant Human Kallikrein 4/KLK4 Protein (His Tag)(Active) 10ug
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89-7755-53
Recombinant Human Kallikrein 4/KLK4 Protein (His Tag)(Active) 20ug
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