89-7754-93 ［取扱停止］Recombinant Human KEAP1/INRF2 Protein (His & GST Tag) 50ug PKSH030945
[Elabscience]
特徴
- 背景：Kelch-like ECH-associated protein 1, also known as cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1 and INRF2, is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed, with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor, which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 85 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_036421.2
- タグ種類：N-His & GST
- アミノ酸配列：Gln2-Cys624
- 遺伝子構造：A DNA sequence encoding the human KEAP1 (NP_036421.2) (Gln2-Cys624) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- 別名（遺伝子名）：INRF2;KEAP-1;KLHL19
- 分子量：97.5 kDa
- 組成：Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37588-Elabscience-PKSH030945.pdf
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89-7754-93
Recombinant Human KEAP1/INRF2 Protein (His & GST Tag) 50ug
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89-7754-94
Recombinant Human KEAP1/INRF2 Protein (His & GST Tag) 100ug
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