89-7753-52 ［取扱停止］Recombinant Human Carbonic Anhydrase 5B/CA5B Protein (His Tag)(Active) 10ug PKSH030856
[Elabscience]
特徴
- 背景：Carbonic anhydrase 5B; also known as carbonate dehydratase VB; carbonic anhydrase VB; CA-VB and CA5B; is a member of the alpha-carbonic anhydrase family. The strongest expression of CA5B / CA-VB is in heart; pancreas; kidney; placenta; lung; and skeletal muscle. It is not expressed in liver. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes; including respiration; calcification; acid-base balance; bone resorption; and the formation of aqueous humor; cerebrospinal fluid; saliva; and gastric acid. CAs show extensive diversity in tissue distribution and in their subcellular localization. CA5B / CA-VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA5A / CA-VA. CA5B / CA-VB has a wider tissue distribution than CA5A / CA-VA; which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. CA5A / CA-VA is activated by histamine; L-adrenaline; L- and D-histidine; and L- and D-phenylalanine. It is inhibited by coumarins; sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt). CA5B / CA-VB is inhibited by coumarins; sulfonamide derivatives such as acetazolamide (AZA); saccharin and Foscarnet (phosphonoformate trisodium salt).
仕様
- サイズ：10ug
- 種由来：Human
- 発現宿主：E.coli
- 純度：> 97 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：Please contact us for more information.
- アクセッションＮｏ．：Q9Y2D0
- タグ種類：C-His
- アミノ酸配列：Cys 34-Pro 317
- 遺伝子構造：A DNA sequence encoding the mature form of human CA5B (Q9Y2D0) (Cys 34-Pro 317) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
- 活性：Measured by its esterase activity. The specific activity is >150 pmoles/min/μg.
- 別名（遺伝子名）：Carbonic Anhydrase 5B Mitochondrial; Carbonate Dehydratase VB; Carbonic Anhydrase VB; CA-VB; CA5B
- 分子量：34 kDa
- 組成：Lyophilized from sterile 20mM Tris; 50mM NaCl; 0.05% Brij-35; pH 8.0
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-105470-Elabscience-PKSH030856.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7753-52
Recombinant Human Carbonic Anhydrase 5B/CA5B Protein (His Tag)(Active) 10ug
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89-7753-53
Recombinant Human Carbonic Anhydrase 5B/CA5B Protein (His Tag)(Active) 20ug
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