特徴
- 背景：Ubiquitin-conjugating enzyme E2 A (also known as HHR6A or UBE2A); encoded by human DNA repair genes HHR6A; belongs to the ubiquitin-conjugating enzymes (E2 enzymes) family and is likely to be involved in postreplication repair and induced mutagenesis. UBE2A is described as a CDK2 substrate. It is the human homologue of the product of the Saccharomyces cerevisiae RAD6 / UBC2 gene; a member of the family of ubiquitin-conjugating enzymes. In vivo; HHR6A phosphorylation peaks during the G2/M phase of cell cycle transition; with a concomitant increase in histone H2B ubiquitylation. Mutation of Ser120 to threonine or alanine abolished UBE2A activity; while mutation to aspartate to mimic phosphorylated serine increased UBE2A activity 3-fold. A mutation of UBE2A is consisdered as the cause of a novel X-linked mental retardation (XLMR) syndrome that affects three males in a two-generation family.
仕様
- サイズ：100ug
- 種由来：Human
- 発現宿主：E.coli
- 純度：> 80 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：Please contact us for more information.
- アクセッションＮｏ．：P49459
- タグ種類：N-His
- アミノ酸配列：Met 1-Cys 152
- 遺伝子構造：A DNA sequence encoding the mature form of human UBE2A (P49459) (Met 1-Cys 152) was expressed; with a polyhistidine tag at the N-terminus.
- 別名（遺伝子名）：Ubiquitin-Conjugating Enzyme E2 A; RAD6 Homolog A; HR6A; hHR6A; Ubiquitin Carrier Protein A; Ubiquitin-Protein Ligase A; UBE2A; RAD6A
- 分子量：19.2 kDa
- 組成：Lyophilized from sterile PBS; 20% glycerol; pH 7.5
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37476-Elabscience-PKSH030787.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
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89-7752-26
Recombinant Human UBE2A Protein (His Tag) 50ug
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89-7752-27
Recombinant Human UBE2A Protein (His Tag) 100ug
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