特徴
- 背景：UBE2H is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1); which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.
仕様
- サイズ：100ug
- 種由来：Human
- 発現宿主：E.coli
- 純度：> 93 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：Please contact us for more information.
- アクセッションＮｏ．：P62256
- アミノ酸配列：Met 1-Leu 183
- 遺伝子構造：A DNA sequence encoding the human UBE2H (P62256) (Met 1-Leu 183) was expressed and purified; with additional two amino acids (Gly & Pro) at the N-terminus.
- 別名（遺伝子名）：Ubiquitin-Conjugating Enzyme E2 H; UbcH2; Ubiquitin Carrier Protein H; Ubiquitin-Conjugating Enzyme E2-20K; Ubiquitin-Protein Ligase H; UBE2H;E2-20K;GID3;UBC8;UBCH;UBCH2
- 分子量：
- 組成：Lyophilized from sterile PBS; 10% glycerol; 2mM DTT; pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37464-Elabscience-PKSH030772.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7752-05
Recombinant Human Ube2H Protein 100ug
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89-7752-04
Recombinant Human Ube2H Protein 50ug
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